Seagrass or submerged aquatic vegetation (SAV) is a valuable and abundant resource found within Gulf Islands National Seashore. It provides habitat for many fish and invertebrates. Seagrass grows in shallow waters and, therefore, is prone to damage and erosion from boats, propellers, fishing equipment, and wakes. We have employed an object-based image analysis technique using Trimble eCognition software to quantify the seagrass and provide a baseline for future studies.